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Image Search Results
Journal:
Article Title: Innate and acquired humoral immunities to influenza virus are mediated by distinct arms of the immune system
doi:
Figure Lengend Snippet: Serum from allotype chimeras contains B-1 and B-2 cell-derived Ig. Concentrations of a- and b-allotype IgM, IgG2a, and IgA in sera of allotype chimeras (n = 13) and controls (for Igha: BALB/c mice, n = 12; for Ighb: C.B-17 mice, n = 11) were determined by ELISA. In chimeras, Igha is PerC donor-derived and therefore produced by B-1 cells (see Figs. Figs.11 and and2),2), and Ighb is host-derived. Data are shown as interquartile boxes; disconnected bars above and below the boxes indicate the 90th and 10th percentiles, respectively. ■, Data from individual mice. ELISA were highly specific, as measurement of sera from the nonrelevant allotype were below the threshold of detection for the assays (<0.01 μg/ml for IgM and IgG2a, <0.001 μg/ml for IgA). Statistical significance was tested with the Wilcoxon Rank test (P < 0.05); n.s., not significant.
Article Snippet: ELISA were developed with biotinylated DS-1; AF6.78; 20.8.3; 5.7 (anti-IgG2a b ), or
Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay, Produced